SPR context
Surface Plasmon Resonance measures binding events at a functionalized sensor surface.
Technical overview
SPR experiments depend on the quality of the ligand layer: orientation, stability and nonspecific background can strongly affect interpretation.
Antibody orientation
Oriented immobilization can help preserve antigen-binding activity compared with random adsorption.
ImmunoLyte positioning
SPR is one of the biosensor platforms supported by the ImmunoLyte workflow.
How it works
Prepare chip
Start from the SPR-compatible metallic surface.
UV-PIT activation
Activate the ImmunoLyte layer using UV.
Immobilize IgG
Build an oriented antibody recognition layer.
Measure kinetics
Run association and dissociation measurements on the prepared surface.
Key benefits
1
Ligand activity
Supports exposed antigen-binding sites.
2
Reproducibility
Useful for repeated chip preparation.
3
Consistent preparation
Supports consistent surface preparation before kinetic measurements.
4
Related workflows
Connects to QCM and general biosensor pages.
Applications
SPR kinetics
Association and dissociation workflows.
Antibody screening
Surface-prepared ligand layers.
Method development
Testing surface preparation parameters.
Diagnostics R&D
Biosensor assay optimization.
Comparative biosensing
SPR paired with QCM or other platforms.
Technical highlights
| Core technology | UV-PIT photo-activated antibody functionalization |
| Main target | IgG antibody activation and oriented immobilization |
| Activation timing | Main ImmunoLyte communication describes IgG activation in about 30 seconds |
| Formats | Metallic surfaces, flat substrates, nanostructures and nanoparticles |
| Relevant platforms | SPR, QCM, magnetoelastic sensors and diagnostic assay development |
| Commercial contact | PRO-COM Alta Tecnologia s.r.l. – procomat@procomat.it |
Functionalization overview
FAQ
Can ImmunoLyte be used in SPR workflows?
Yes. SPR is one of the biosensor platforms described for ImmunoLyte functionalization workflows.
Why mention orientation for SPR?
Because ligand orientation can influence accessibility and signal behavior.
Which related ImmunoLyte workflows are relevant to SPR?
Related workflows include antibody functionalization, UV-PIT activation, QCM functionalization and general biosensor surface preparation.
Explore related ImmunoLyte topics
Continue with related ImmunoLyte workflows and application areas.
Ready to discuss SPR Functionalization?
Contact PRO-COM Alta Tecnologia s.r.l. for technical information, availability and commercial details.